The Effect of Insulin-like Growth Factor-binding Protein-3 On
نویسندگان
چکیده
To determine if insulin-like growth factor binding protein-3 (IGFBP-3) can directly modulate the hormone-dependent steroidogenesis of thecal cells in vitro, thecal cells from large (≥ 8 mm) follicles were collected from cattle and cultured for 4 d. IGFBP-3 inhibited IGF-I-induced thecal cell progesterone and androstenedione production by 52% and 89%, respectively. In contrast, IGFBP-3 had no effect (P>.10) on LH-induced progesterone or androstenedione production by thecal cells. Higher doses of IGF-I overcame the inhibitory effects of IGFBP-3 on IGF-I-induced progesterone and androstenedione production by thecal cells. IGFBP-3 directly inhibited I-IGF-I binding to thecal cells. We conclude that IGFBP-3 may play an important role in regulating thecal cell steroidogenesis during follicular development in cattle.
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